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KMID : 0382320060260040125
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Environmental Mutagens and Carcinogens
2006 Volume.26 No. 4 p.125 ~ p.132
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Genome-based Gene Expression Analysis of EGCG-mediated Cell Transformation Suppression Effect in Mouse Cell line Balb/c 3T3 A31-1-1
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Jung Ki-Kyung
Park Sue-Nie
Kim Seung-Hee
Kim Tae-Gyun
Jung Hai-Kwan
Suh Soo-Kyung
Park Moon-Suk
Lee Woo-Sun
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Abstract
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Previous studies showed that epigallocatechin gallate (EGCG) have substantial effects of suppressing the N-methyl-N’-nitro-N-nitrosoguanidine (MNNG)-initiated cell transformation process on the bases of foci formation frequency and loss of anchorage dependency. In this study we tried to clarify the molecular mechanism of suppressing the cell transformation process. Mouse cell line balb/c 3T3 A31-1-1 was exposed 2 days to MNNG followed by 15 days 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment for our transformation process. EGCG was added after the time point of 24 hours exposure to TPA and incubated for 19 days. 2029 genes were selected in our transformation process that showed fold change value of 1.5 or more in the microarray gene expression analysis covering the mouse full genome. These genes were found to be involved mainly in the cell cycle pathway, focal adhesion, adherens junction, TGF-β signaling, apoptosis, lysine degradation, insulin signaling, ECM-receptor interaction. Among the genes, we focused on the 631 genes (FC ＞ 0.5) reciprocally affected by EGCG treatment. Our study suggest that EGCG down-regulate the gene expressions of up stream signaling factors such as nemo like kinase with MAPK activity and PI3-Kinase, Ras GTPase and down stream factors such as cyclin D1, D2, H, T2, cdk6.
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KEYWORD
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Cell transformation, MNNG, carcinogenesis, microarray, EGCG
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